Compositional Control of Phase-Separated Cellular Bodies

Compositional Control of Phase-Separated Cellular Bodies

Cellular bodies such as P bodies and PML nuclear bodies (PML NBs) appear to be phase-separated liquids organized by multivalent interactions among proteins and RNA molecules. Although many components of various cellular bodies are known, general principles that define body composition are lacking. We modeled cellular bodies using several engineered multivalent proteins and RNA. In vitro and in ...

Cellular response to phase-separated blends of tyrosine-derived polycarbonates.

Two-dimensional thin films consisting of homopolymer and discrete compositional blends of tyrosine-derived polycarbonates were prepared and characterized in an effort to elucidate the nature of different cell responses that were measured in vitro. The structurally similar blends were found to phase separate after annealing with domain sizes dependent on the overall composition. The thin polymer...

SnapShot: Cellular Bodies

Cajal Body 0–6 0.1–2.0 µm; round Coilin Involved in snRNP and snoRNP biogenesis and posttranscriptional modification of newly assembled spliceosomal snRNAs. Clastosome 0–3 0.2–1.2 µm; irregular 20S core catalytic component of proteasome Contains ubiquitin conjugates, the proteolytically active 20S core and 19S regulatory complexes of the 26S proteasome, and protein substrates of the proteasome.

Nanostructures from Phase Separated Polymers

Adaptive Control of Separated Flow

Adaptive closed-loop control is used to optimize the lift-to-drag ratio of post-stall separated flow over a NACA 0025 airfoil using multiple amplitudemodulated (AM) or burst-modulated (BM), zero-net-mass-flux (ZNMF) actuators that cover the central 33% span of the airfoil. A simplex optimization approach uses the lift and drag measured by a strain-gauge balance for feedback and searches for the...